Human EGFR/ERBB1/HER1 Recombinant Protein (N-His)
- Catalog number: PH15679
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human EGFR/ERBB1/HER1 Protein, N-His (PH15679) expressed in E. coli, spanning Leu25-Ser151. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Leu25-Ser151
- Predicted molecular weight: 16.87 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: HER1, Receptor tyrosine-protein kinase erbB-1, Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGFR, ERBB1, ERBB
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix).
References:
1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725
2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536
3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 279096
4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033
5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049
6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079
7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029
8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704 - Note: For research use only.
Images
-
SDS-PAGESDS-PAGE for Recombinant Human EGFR/ERBB1/HER1 Protein, N-His
