Human CD368/CLEC4D Recombinant Protein (N-His)
- Catalog number: PH15976
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human CD368/CLEC4D Protein, N-His (PH15976) expressed in E. coli, spanning Cys39-Asn215. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Cys39-Asn215
- Predicted molecular weight: 22.95 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: C-type lectin domain family 4 member D, C-type lectin superfamily member 8, C-type lectin-like receptor 6, CLEC-6, Dendritic cell-associated C-type lectin 3, DC-associated C-type lectin 3, Dectin-3, CD368, CLEC4D, CLECSF8, MCL
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: C-type lectin domain family 4 member D (CD368/CLEC4D) is a ~24 kDa protein. Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of pathogen-associated molecular patterns (PAMPs) of bacteria and fungi. The PAMPs include alpha-mannans on C.albicans hypheas and mycobacterial trehalose 6,6'-dimycolate (TDM). Interacts with signaling adapter Fc receptor gamma chain/FCER1G, likely via CLEC4E, to form a functional complex in myeloid cells. Binding of mycobacterial TDM or C.albicans alpha-mannans to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. The heterodimer formed with CLEC6A is active against fungal infection.
References:
1. Miyake, Y. et al. (2013) Immunity 38, 1050-62. PMID: 23602766
2. Zhu, LL. et al. (2013) Immunity 39, 324-34. PMID: 23911656
3. Arce, I. et al. (2004) European journal of immunology 34, 210-20. PMID: 14971047 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human CD368/CLEC4D Protein, N-His
