Human HTR2C Recombinant Protein (N-His-KSI)
- Catalog number: PH16268
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human HTR2C Protein, N-His-KSI (PH16268) expressed in E. coli, spanning Leu236-Lys311. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Leu236-Lys311
- Predicted molecular weight: 24.62 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: 5-hydroxytryptamine receptor 2C, 5-HT-2C, 5-HT2C, 5-HTR2C, 5-hydroxytryptamine receptor 1C, 5-HT-1C, 5-HT1C, Serotonin receptor 2C, HTR2C, HTR1C
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: 5-hydroxytryptamine receptor 2C (HTR2C) is a ~51 kDa protein. G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. HTR2C is coupled to G(q)/G(11) G alpha proteins and activates phospholipase C-beta, releasing diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) second messengers that modulate the activity of phosphatidylinositol 3-kinase and promote the release of Ca(2+) ions from intracellular stores, respectively. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways.
References:
1. Schaerlinger, B. et al. (2003) British journal of pharmacology 140, 277-84. PMID: 12970106
2. Cussac, D. et al. (2008) European journal of pharmacology 594, 32-8. PMID: 18703043
3. Knauer, CS. et al. (2009) Naunyn-Schmiedeberg's archives of pharmacology 379, 461-71. PMID: 19057895
4. Peng, Y. et al. (2018) Cell 172, 719-730.e14. PMID: 29398112
5. Stam, NJ. et al. (1994) European journal of pharmacology 269, 339-48. PMID: 7895773 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human HTR2C Protein, N-His-KSI
