Human TRAM1 Recombinant Protein (N-His-KSI)
- Catalog number: PH16518
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human TRAM1 Protein, N-His-KSI (PH16518) expressed in E. coli, spanning Phe318-Ser374. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Phe318-Ser374
- Predicted molecular weight: 22.09 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: TRAM, TRAM1, Translocating chain-associated membrane protein 1
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Translocating chain-associated membrane protein 1 (TRAM1) is a ~43 kDa protein. Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating the proper chain positioning at the SEC61 channel. Regulates the exposure of nascent secretory protein chain to the cytosol during translocation into the ER. May affect the phospholipid bilayer in the vicinity of the lateral gate of the SEC61 channel, thereby facilitating ER protein transport. Intimately associates with transmembrane (TM) domain of nascent membrane proteins during the entire integration process into the ER membrane. Associates with the second TM domain of G-protein-coupled receptor opsin/OPSD nascent chain in the ER membrane, which may facilitate its integration into the membrane.
References:
1. Meacock, SL. et al. (2002) Molecular biology of the cell 13, 4114-29. PMID: 12475939
2. Görlich, D. et al. (1992) Nature 357, 47-52. PMID: 1315422
3. Klein, MC. et al. (2020) Channels (Austin, Tex.) 14, 28-44. PMID: 32013668
4. Do, H. et al. (1996) Cell 85, 369-78. PMID: 8616892
5. Hegde, RS. et al. (1998) Cell 92, 621-31. PMID: 9506517
6. Ng, CL. et al. (2010) Experimental cell research 316, 2113-22. PMID: 20430023 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human TRAM1 Protein, N-His-KSI
