Mouse GSDMD/Gasdermin-D Recombinant Protein (N-His-SUMO)
- Catalog number: PM10287
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Mouse GSDMD/Gasdermin-D Protein, N-His-SUMO (PM10287) expressed in E. coli, spanning Met1-Asp276. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Asp276
- Predicted molecular weight: 42.87 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Gasdermin-D, Gasdermin domain-containing protein 1, Gasdermin-D, N-terminal, GSDMD-NT, mGSDMD-NTD, p30, Gasdermin-D, C-terminal, GSDMD-CT, mGSDMD-CTD, p20, Gsdmd, Gsdmdc1
- Species: Mus musculus (Mouse)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Gasdermin-D (GSDMD) is a ~53 kDa protein. Precursor of a pore-forming protein that plays a key role in host defense against pathogen infection and danger signals. This form constitutes the precursor of the pore-forming protein: upon cleavage, the released N-terminal moiety (Gasdermin-D, N-terminal) binds to membranes and forms pores, triggering pyroptosis.
References:
1. Shi, J. et al. (2015) Nature 526, 660-5. PMID: 26375003
2. Kayagaki, N. et al. (2015) Nature 526, 666-71. PMID: 26375259
3. He, WT. et al. (2015) Cell research 25, 1285-98. PMID: 26611636
4. Liu, X. et al. (2016) Nature 535, 153-8. PMID: 27383986
5. Russo, HM. et al. (2016) Journal of immunology (Baltimore, Md. : 1950) 197, 1353-67. PMID: 27385778
6. Sborgi, L. et al. (2016) The EMBO journal 35, 1766-78. PMID: 27418190 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Mouse GSDMD/Gasdermin-D Protein, N-His-SUMO
